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rat monoclonal anti cd68  (Bio-Rad)


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    Structured Review

    Bio-Rad rat monoclonal anti cd68
    Rat Monoclonal Anti Cd68, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 3144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd68+monoclonal+antibody/Rat+anti+Mouse+CD68/pm42811006-544-43-47
    Average 96 stars, based on 3144 article reviews
    rat monoclonal anti cd68 - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Generated:

    Article Title: Seeking rhythmic patterns in microglial cells within the circadian pineal gland from male rats
    Article Snippet: Images were captured using an Olympus FV1000 confocal microscope (Olympus America Inc., Center Valley, USA; RRID:SCR_020337), and processed with the open-source Fiji software ( https://fiji.sc/ ; RRID:SCR_002285). .. The anti-CD68 monoclonal antibody generated in mice (Bio-Rad, clone ED1, Cat# MCA341GA) was raised against the rat CD68 (cluster of differentiation 68) protein derived from spleen cells. ..

    Derivative Assay:

    Article Title: Seeking rhythmic patterns in microglial cells within the circadian pineal gland from male rats
    Article Snippet: Images were captured using an Olympus FV1000 confocal microscope (Olympus America Inc., Center Valley, USA; RRID:SCR_020337), and processed with the open-source Fiji software ( https://fiji.sc/ ; RRID:SCR_002285). .. The anti-CD68 monoclonal antibody generated in mice (Bio-Rad, clone ED1, Cat# MCA341GA) was raised against the rat CD68 (cluster of differentiation 68) protein derived from spleen cells. ..

    Incubation:

    Article Title: PHARMACOLOGICAL INHIBITION OF INTEGRIN ALPHAV BETA3 AGGRAVATES EXPERIMENTAL LIVER FIBROSIS AND SUPPRESSES HEPATIC ANGIOGENESIS
    Article Snippet: .. Anti-CD68 monoclonal antibody (Serotec, Kidlington, UK) was applied at a 1:100 dilution overnight at +4°C, following by incubation with horseradish peroxidase-conjugated goat anti-mouse antibody (1:2000). .. Immunodetected proteins were visualized using enhanced chemiluminescence (Amersham Biosciences, Freiburg, Germany).

    Immunohistochemistry:

    Article Title: A DARPin targeting activated Mac-1 is a novel diagnostic tool and potential anti-inflammatory agent in myocarditis, sepsis and myocardial infarction
    Article Snippet: .. Immunohistochemistry was performed using an anti-CD68 monoclonal antibody (FA-11, 50 μl per slide, 1:500 dilution; 30 min, RT; Bio-Rad, USA) or an isotype control (Invitrogen, USA). .. Slides were then incubated with a secondary anti-rat-biotin-conjugated antibody (50 μl per slide, 1:200, 30 min, RT; Vector Labs, United Kingdom).

    Control:

    Article Title: A DARPin targeting activated Mac-1 is a novel diagnostic tool and potential anti-inflammatory agent in myocarditis, sepsis and myocardial infarction
    Article Snippet: .. Immunohistochemistry was performed using an anti-CD68 monoclonal antibody (FA-11, 50 μl per slide, 1:500 dilution; 30 min, RT; Bio-Rad, USA) or an isotype control (Invitrogen, USA). .. Slides were then incubated with a secondary anti-rat-biotin-conjugated antibody (50 μl per slide, 1:200, 30 min, RT; Vector Labs, United Kingdom).



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    Validation of CXCL9 + and SPP1 + macrophage expression and polarization characteristics in lung cancer tissues. (A) Representative mIF staining for CXCL9+ TAMs and SPP1+ TAMs in lung tissue sections from the normal and LUAD tumor groups. DAPI (blue), <t>CD68</t> (red), CXCL9 (yellow), SPP1 (green) are shown, along with individual and merged channels. (n = 3 per group). Scale bar, 20 μm. (B) Representative mIF staining of CXCL9+ TAMs (yellow) and SPP1+ TAMs (green) in lung tissue sections from the normal and LUAD tumor groups (n = 3 per group). Scale bar, 20 μm. (C) qRT-PCR validation of macrophage polarization. The M1 group showed high expression of Cxcl9 and iNOS, while the M2 group showed high expression of Spp1 and Arg1. Asterisks indicate statistically significant differences (*p < 0.05).
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    Validation of CXCL9 + and SPP1 + macrophage expression and polarization characteristics in lung cancer tissues. (A) Representative mIF staining for CXCL9+ TAMs and SPP1+ TAMs in lung tissue sections from the normal and LUAD tumor groups. DAPI (blue), <t>CD68</t> (red), CXCL9 (yellow), SPP1 (green) are shown, along with individual and merged channels. (n = 3 per group). Scale bar, 20 μm. (B) Representative mIF staining of CXCL9+ TAMs (yellow) and SPP1+ TAMs (green) in lung tissue sections from the normal and LUAD tumor groups (n = 3 per group). Scale bar, 20 μm. (C) qRT-PCR validation of macrophage polarization. The M1 group showed high expression of Cxcl9 and iNOS, while the M2 group showed high expression of Spp1 and Arg1. Asterisks indicate statistically significant differences (*p < 0.05).
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    Validation of CXCL9 + and SPP1 + macrophage expression and polarization characteristics in lung cancer tissues. (A) Representative mIF staining for CXCL9+ TAMs and SPP1+ TAMs in lung tissue sections from the normal and LUAD tumor groups. DAPI (blue), <t>CD68</t> (red), CXCL9 (yellow), SPP1 (green) are shown, along with individual and merged channels. (n = 3 per group). Scale bar, 20 μm. (B) Representative mIF staining of CXCL9+ TAMs (yellow) and SPP1+ TAMs (green) in lung tissue sections from the normal and LUAD tumor groups (n = 3 per group). Scale bar, 20 μm. (C) qRT-PCR validation of macrophage polarization. The M1 group showed high expression of Cxcl9 and iNOS, while the M2 group showed high expression of Spp1 and Arg1. Asterisks indicate statistically significant differences (*p < 0.05).
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    Bio-Rad rat monoclonal
    Validation of CXCL9 + and SPP1 + macrophage expression and polarization characteristics in lung cancer tissues. (A) Representative mIF staining for CXCL9+ TAMs and SPP1+ TAMs in lung tissue sections from the normal and LUAD tumor groups. DAPI (blue), <t>CD68</t> (red), CXCL9 (yellow), SPP1 (green) are shown, along with individual and merged channels. (n = 3 per group). Scale bar, 20 μm. (B) Representative mIF staining of CXCL9+ TAMs (yellow) and SPP1+ TAMs (green) in lung tissue sections from the normal and LUAD tumor groups (n = 3 per group). Scale bar, 20 μm. (C) qRT-PCR validation of macrophage polarization. The M1 group showed high expression of Cxcl9 and iNOS, while the M2 group showed high expression of Spp1 and Arg1. Asterisks indicate statistically significant differences (*p < 0.05).
    Rat Monoclonal, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Validation of CXCL9 + and SPP1 + macrophage expression and polarization characteristics in lung cancer tissues. (A) Representative mIF staining for CXCL9+ TAMs and SPP1+ TAMs in lung tissue sections from the normal and LUAD tumor groups. DAPI (blue), CD68 (red), CXCL9 (yellow), SPP1 (green) are shown, along with individual and merged channels. (n = 3 per group). Scale bar, 20 μm. (B) Representative mIF staining of CXCL9+ TAMs (yellow) and SPP1+ TAMs (green) in lung tissue sections from the normal and LUAD tumor groups (n = 3 per group). Scale bar, 20 μm. (C) qRT-PCR validation of macrophage polarization. The M1 group showed high expression of Cxcl9 and iNOS, while the M2 group showed high expression of Spp1 and Arg1. Asterisks indicate statistically significant differences (*p < 0.05).

    Journal: Frontiers in Immunology

    Article Title: The CXCL9/SPP1 polarity axis in tumor-associated macrophages: immunoregulatory and prognostic significance in non-small cell lung cancer

    doi: 10.3389/fimmu.2026.1763652

    Figure Lengend Snippet: Validation of CXCL9 + and SPP1 + macrophage expression and polarization characteristics in lung cancer tissues. (A) Representative mIF staining for CXCL9+ TAMs and SPP1+ TAMs in lung tissue sections from the normal and LUAD tumor groups. DAPI (blue), CD68 (red), CXCL9 (yellow), SPP1 (green) are shown, along with individual and merged channels. (n = 3 per group). Scale bar, 20 μm. (B) Representative mIF staining of CXCL9+ TAMs (yellow) and SPP1+ TAMs (green) in lung tissue sections from the normal and LUAD tumor groups (n = 3 per group). Scale bar, 20 μm. (C) qRT-PCR validation of macrophage polarization. The M1 group showed high expression of Cxcl9 and iNOS, while the M2 group showed high expression of Spp1 and Arg1. Asterisks indicate statistically significant differences (*p < 0.05).

    Article Snippet: Following another stripping step, the third round was performed using mouse anti-human CD68 monoclonal antibody (1:50000, Servicebio, Cat# GB153150 ) with HRP-conjugated goat anti-mouse secondary antibody (ready-to-use, Servicebio, Cat# G1301), and signal was developed using iF555-Tyramide (1:500, Servicebio, Cat# G1233).

    Techniques: Biomarker Discovery, Expressing, Staining, Quantitative RT-PCR